The primary goals of the fabrication and operation of artificial organelles are to create reaction vessels for the construction of biologically significant products and to employ such a platform to gain a fundamental understanding of organelle structure and function. Digital microfluidics facilitates open droplet movement across a 2D grid-like surface by the process of electrowetting. This technology has opened up new opportunities to design nanoscale biomimetic droplet vesicle-based reaction systems. These systems can be used to mimic the natural fluid vesicles that operate in organelles. Using digital microfluidics, recombinant enzyme technology, and magnetic nanoparticles, we have created a functional prototype of an artificial Endoplasmic reticulum and the Golgi organelles. These artificial organelles allow for in vitro protein core biosynthesis followed by enzymatically modification of glycosaminoglycans analogous to biosynthesis in eukaryotic cells.